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mrdizee

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  1. Grassmatch, interesting post on mitotoc inhibitors. Probably not something I'll do for a while, if ever, but fascinating, nonetheless. I remember hybrid corn we used to plant on the farm back before GMOs were the thing...the seeds weren't fertile. That article explains it (I think). Good luck with your pursuits. Yeah, I've read about the gene gun in a few different places...hell I've got to get a culture through to full plant status! I've only messed with cannabis culture except for taking a couple cuttings off of my wife's bonsai tree. They are doing well so far. It would probably be beneficial to do some other things to put a bit more fun into it. I do have my eye on one of my neighbor's camellia bushes that blossoms prolifically...I mean over the top solid red every spring. Going to snatch some from that as soon as it starts shooting this spring. There are no others in this area doing tissue culture that I'm aware of. I've spoken with enough to folks that if there were others pursuing this I would have heard through the grapevine. I'm talking once in a while with a guy in CA who has had good luck...he's been doing TC and hydro for decades. You probably know him, or know of him. Will probably take a drive down there at some point to see what he's up to. The only real connection I've had with others in this field is on these forums, and most, I've found out, are not very genuine. If I can perfect this process, I'll have more business than I can handle. There are two growers I'm in with that will build a lab when that happens. Each of them will be growing several thousand plants a year of legal rec, via indoor grows. Medicinal grows are going to be going away because the state is trying to push it out...they cannot tax it. Medicinal varieties will still be grown and sold to dispensaries but not under a medicinal license. A grower can only have one or the other...medicinal or rec. I'm also loosely associated with another group of several growers. The one thing they all have in common is they want CLEAN starts with no PM or russet mites. TC can offer that without dousing them in chemicals. Personally, I have no interest in growing...only TC.
  2. Grassmatch, just to clarify, the callus I'm talking about that grew the leaves is in the photo I posted On Feb 16, not my profile photo. My profile photo was an explant put directly into shoot generation formula about three weeks prior and then transferred to rooting formula; still waiting for it to root.
  3. I originally took the leaf cutting last October and have divided the callus several times. Once I put it into the container you see in the photo it took a couple weeks for the leaves to show up. Yesterday I put it into rooting formula. Attached is another piece of callus that was actually in a shoot formula, but just keeps getting larger. After a month it has grown to present size from a pea size. Going to take a sample and put it into another shoot formula to see if I can get it to respond in a favorable way.
  4. My plants just don't seem to be real vigorous for the most part and am wondering if it's a water issue. I'm using distilled water, but most studies I have read say to use double distilled de-ionized water. Any thoughts?
  5. Grassmatch, I'm thinking of getting one of those ultrasonic cleaners that you had recommend in the thread. If I normally soak my explants in bleach for, say, 10 minutes, should I use the same concentration and times in the ultrasonic, or shorter? I don't have much contamination issue, 10% maybe, and would like to pare that down a bit.
  6. Thanks for the tips...gives me some other options. I am curious what you mean by "shaven." Do you shave the outer layer off of the stem butt to expose a different type of tissue? My explants usually have a sort of bulbous formation on the base of the stem.
  7. The leaves coming out of the callus are regenerated from the callus, not from a cutting. The original cutting was just a small section of leaf that grew into the callus from which the new leaves came. They are very slow growing at this point and it's the only piece of callus that has grown leaves. The other calli just sit there, expanding, some green, some yellow. So, the idea with the battery/LED in the envelope would be for future shipping purposes? How long does it generally take for enough roots to form to move the explant into soil? How do you release the agar from the roots without damaging them? I haven't tried it yet, but the looks so fragile.
  8. I've got about 20 jars with this strain of callus in them, this one is the most advanced but many others are starting to get little green dots in them, so I've got plenty to work with. This particular one also looks as if some rooting may be in the works. The formula, taken from a study, was supposed to be for callus induction and growth, but the same cytokinin/auxin ratio and ingredients are also used (in another study) for shoot induction from callus as well as a multiplication medium.
  9. Sounds like a lot of folks want to be able to drive stoned. I think that's a really bad idea, regardless of any laws that are, or are not in place. Driving a 2000 to 10,000 lbs (or larger) vehicle under the influence of any substance, legal or not, prescription or not, is not a good thing. I'm all for the legalization of cannabis, in all its forms, but there has to be some responsibility on the part of the user to protect the general public as well as your own behind. Perhaps the laws are a bit restrictive, but you can always change your behaviors to be a safe responsible user. 1. I don't know what it's like in MI but in OR the dispensaries don't smell like pot. All herb is in closed containers so there would be no reason for your clothes to smell like pot. 2. Why would you be pulled over for a minor traffic violation? Are you stoned? Are you driving stupid? If so, you shouldn't be driving in the first place. For gosh sakes, put your card in a different place in your wallet where the officer can't see it...sheesh!!! 3. If it's been a "few hours" since you smoked, you wouldn't smell like pot. Plan a little better...don't go shopping if you've smoked. Supposed you downed a fifth of whiskey a "few hours" ago...would you drive then? If so, you deserve to be arrested. And, how often does your car break down, anyway? I've been driving for 50 years and have only had two breakdowns in that time and a cop didn't show up either time. I think you're viewing this from an alarmist perspective; your logic needs a bit of work. If you want to change the law then work towards changing it so everyone can drive stoned with no repercussions. Is that what you want? People shouldn't drive drunk, they shouldn't drive stoned, and they shouldn't drive under the influence of any mind/mood altering substances, even prescribed by a doctor. Be responsible.
  10. Organogenesis from callus tissue of a high CBD medicinal plant. Funny thing is, this is happening on a formula for expanding callus, not from a shoot formula, containing both an auxin and cytokinin. Going to try some formulas containing only an auxin and only a cytokinin. Will I get only roots, or only shoots? I don't know, but it will be fun trying!
  11. Found some roots growing on this plantlet today. It was sort of stalled in it's rooting medium but took off a couple days ago, growing very well and today there is at least one root showing...two weeks to the day. Hope the rest follow suit. It seems like every step of this process is a challenge. To complicate matters it also seems like every variety responds differently to culture conditions. At any rate, the next challenge is hardening off, from which I understand, can be quite the art-form.
  12. Actually I didn't know what I was doing when I did those first cultures. I just took some leaf material and stuck it in a dish with the stage 1 media, then to the stage 2 media from TriQ systems. I will say that their s-2 grows callus very well. However, due to different issues with their media I didn't continue using it. Realized it was much less expensive to make my own (which I do for about 3 bucks a liter). So I had to re-learn chemistry that I slept though in HS when I was stoned, lol. It's been fun, getting the "aha moments" when one connects the dots. Having a biochemist as my next door neighbor has helped a lot, too! Haven't really tried growing much callus lately, although I do have a good recipe for it. One study I read found that the petioles work best for callus and will be trying that in the near future after I get my moms cleaned up. I've noticed callus growing at the base of nodes that I put into culture, but have thought that was a detrimental thing. After reading about carrot TC, perhaps it is not. Here's my situation, presently, and it's what got me into TC in the first place. A friend, who's a grower approached me because he knew I did mushroom culture. (I know I'm repeating part of this story, but bear with me). He had two small moms that had started flowering and wanted to save the genetics. The Z7 ant the QTZ. Being full of trichomes, they were also full of contamination. I took the part that wrorked the best initially...the leaves. Later I was able to coax the plant back to veg state and got some good nodes to culture. Still have some of those stable in culture but fear I will lose them. Some of them look healthy enough to clip some petioles so I can make callus and get it to shoot and root at a later time when I'm a bit more accomplished at his art from. Since then I've been approached by several growers for clean starts. If I could produce them, I could have orders for several thousand starts. Of course, I don't have the technique or space for such an operation. However, the guy who initially approached me is willing to build a lab for such an operation, as well as one other person, too. I'd like this to be my "second career" as I approach retirement age. I'm a bit shocked that it took so long for you to arrive at a suitable protocol; it's also a bit discouraging. But, I will keep at it, nonetheless. I understand I have many more resources at my disposal than you had for many of your years and I have a lot of determination, as well as humility because I don't have the background many others in this business have. BTW, it won't be anytime soon that I go off of PPM, just something I think about. I want to simplify things as much as possible, and perhaps PPM allows that, for now. I really appreciate having someone to talk with about this stuff...thanks!
  13. Most of the callus I've got was from leaf tissue. It was the first TC I tried doing last Sept. Regarding the russet mites, I guess I need to explain a bit better. I have a couple small moms that I was getting cuttings from for experimenting...someone had given them to me. I think one of them brought the mites in and infected the other one, as well as a couple more plants that came and went. The photos I posted showed the leaves of one of the moms. The photo of the culture jar was of one of my cultures that I had thought was infected, too. I think I pushed the panic button on thinking the culture jars were infected, but they are not. The mites I saw were on the live mom and definitely alive. I've come to believe the curling of the leaves in vitro is from vitrification and I'm going to approach a solution from that angle. I'm going to use a different cytokinin for starters and see how that works; perhaps cut back on the salts. Sometimes less is more, as they say. Regardless of any issues with mites, or not, I'm wrapping my jars from now on and will try to wean myself off of PPM.
  14. I don't blame them for not sharing, either. Makes me wonder if there is something intentionally left out of what is published in the studies. The callus is often very delicate and crumbly, like trying to pick up a glob of cooked rice. To quote from Plants from Test Tubes: "Callus can be friable or firm textured and it can be green, cream, yellowish, or brown colored." It seems to vary with media composition and plant variety.
  15. 20 bucks at the local grow shop and works surprisingly well.
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